研究業績Publications

Front Vet Sci. 2025 Jul 10;12:1570720. doi: 10.3389/fvets.2025.1570720.
Enhanced sensitivity, robust p21 activation, and sustained DNA repair responses to interstrand crosslinks in elephant cells compared to humans
Kitano T, Zhu Z, Minami N, Orino K. Yoshikawa Y.

J Vet Med Sci. 2024 May 6;86(5):485-492.doi: 10.1292/jvms.24-0008. Epub 2024 Apr 3.
Searching for new molecules involved in Anopheles mosquitoes' response to Plasmodium infection
Haraguchi A, Takano M, Fujiwara K, Hakozaki J, Nakayama K, Nakamura S, Yoshikawa Y, Fukumoto S, Kusakisako K, Ikadai H.

Front Neurosci. 2023 Oct 18:17:1273039.doi: 10.3389/fnins.2023.1273039. eCollection 2023.
Short-term memory impairment following recovery from systemic inflammation induced by lipopolysaccharide in mice
Morimoto K, Watanuki S, Eguchi R, Kitano T, Otsuguro KI.

Austral Journal of Veterinary Sciences. 2023 Sep doi: 10.4206/ajvs.553.06
Comparisons of β2-microglobulin, apolipoprotein A1, and immunoglobulins (IgG and IgM) detected in the serum and urine from individual cats
Katagi M, Imatomi H, Tsuchida M, Katayama K, Yoshikawa Y, Orino K

In Vivo. 2023 Sep-Oct;37(5):2100-2104.doi: 10.21873/invivo.13307.
Acupuncture Treatment Improves Transport Stress in Microminipigs Through the Acupoint in Ears
Ijiri M, Akioka K, Kitano T, Miura H, Ono HK, Terashima R, Fujimoto Y, Matsuo T, Yamato O, Kawaguchi H.

J Vet Med Sci. 2023 Sep 1;85(9):921-928.doi: 10.1292/jvms.23-0099. Epub 2023 Jul 5.
Formation of free oocysts in Anopheles mosquitoes injected with Plasmodium ookinetes
Haraguchi A, Takano M, Hakozaki J, Nakayama K, Nakamura S, Yoshikawa Y, Fukumoto S, Kusakisako K, Ikadai H.

Cell Rep. 2023 Jul 25;42(7):112721. doi: 10.1016/j.celrep.2023.112721. Epub 2023 Jun 30.
Phosphorylation by ATR triggers FANCD2 chromatin loading and activates the Fanconi anemia pathway
Kupculak M, Bai F, Luo Q, Yoshikawa Y, Lopez-Martinez D, Xu H, Uphoff S, Cohn MA.

Vet Sci. 2023 Feb 10;10(2):145.doi: 10.3390/vetsci10020145.
A Highly Conserved Region in BRCA2 Suppresses the RAD51-Interaction Activity of BRC Repeats
Zhu Z, Kitano T, Morimatsu M, Ochiai K, Ishiguro-Oonuma T, Oosumi K, Lin X, Orino K, Yoshikawa Y.

Vet Sci. 2022 Dec 17;9(12):703.doi: 10.3390/vetsci9120703.
Canine Mammary Tumor Cell Lines Derived from Metastatic Foci Show Increased RAD51 Expression but Diminished Radioresistance via p21 Inhibition
Shimakawa K, Ochiai K, Hirose S, Tanabe E, Michishita M, Sakaue M, Yoshikawa Y, Morimatsu M, Tajima T, Watanabe M, Tanaka Y.

Parasit Vectors. 2022 Jul 8;15(1):244. doi: 10.1186/s13071-022-05357-w.
Plasmodium berghei Brca2 is required for normal development and differentiation in mice and mosquitoes.
Yasunaga Y, Shunta K, Akira S, Makoto S, Aki U, Hiroki K, Zida Z, Kazuhiko O, Kazuhiko N, Jun H, Kodai K, Asako H, Taisuke K, Koichi O, Shinya F, Hiromi I.

Int J Mol Sci. 2022 Apr 6;23(7):4060. doi: 10.3390/ijms23074060.
BRCA2 C-Terminal RAD51-Binding Domain Confers Resistance to DNA-Damaging Agents.
Zida Z, Taisuke K, Masami M, Arisa T, Ryo M, Xianghui L, Koichi O, Yasunaga Y.

BMC Mol Cell Biol. 2021 Jan 6;22(1):2. doi: 10.1186/s12860-020-00336-4.
Reduced translation efficiency due to novel splicing variants in 5' untranslated region and identification of novel cis-regulatory elements in canine and human BRCA2.
Yoshikawa Y, Kozuma H, Morimatsu M, Sugawara K, Orino K.

J Vet Med Sci. 2021 May 9;83(5):759-766. doi: 10.1292/jvms.21-0006.
Identification of the core motif of the BRCA2 C-terminal RAD51-binding domain by comparing canine and human BRCA2.
Yoshikawa Y, Morimatsu M, Ochiai K, Ishiguro-Oonuma T, Morioka R, Okuda K, Orino K.

International Dairy Journal. 2020;105:104690.
Binding analysis of bovine milk proteins, especially casein interactions and the interaction between α-casein and lactoferrin, using beads immobilized with zinc ion and poly-L-lysine or α-casein.
Naito K, Iio T, Katagi M, Yoshikawa Y, Ohtsuka H, Orino K.

Vet Comp Oncol. 2020 Jun;18(2):247-255. doi: 10.1111/vco.12542.
The canine RAD51 mutation leads to the attenuation of interaction with PALB2.
Uemura M, Ochiai K, Morimatsu M, Michishita M, Onozawa E, Azakami D, Uno Y, Yoshikawa Y, Sasaki T, Watanabe M, Omi T.

Cell Rep. 2019 Jun 4;27(10):2990-3005.e5. doi: 10.1016/j.celrep.2019.05.003.
Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage.
Lopez-Martinez D, Kupculak M, Yang D, Yoshikawa Y, Liang CC, Wu R, Gygi SP, Cohn MA.

Biometals. 2018 Feb;31(1):131-138. doi: 10.1007/s10534-017-0075-1.
Heme-binding of bovine lactoferrin: the potential presence of a heme-binding capacity in an ancestral transferrin gene.
Saito N, Iio T, Yoshikawa Y, Ohtsuka H, Orino K.

PLoS Genet. 2018 Oct 18;14(10):e1007643. doi: 10.1371/journal.pgen.1007643.
Identification of UHRF2 as a novel DNA interstrand crosslink sensor protein.
Motnenko A, Liang CC, Yang D, Lopez-Martinez D, Yoshikawa Y, Zhan B, Ward KE, Tian J, Haas W, Spingardi P, Kessler BM, Kriaucionis S, Gygi SP, Cohn MA.

EMS Vet Sci J. 2017;1(1):1.
Changes in anti-Ferritin autoantibody during pregnancy in rats.
Hasegawa R, Kiyota H, Kurusu S, Yoshikawa Y, Orino K.

Biometals. 2017 Aug;30(4):615-621. doi: 10.1007/s10534-017-0032-z.
Inhibitory effect of transferrin on serum zinc measurement.
Orino K.

Biometals. 2016 Feb;29(1):15-24. doi: 10.1007/s10534-015-9897-x.
Physiological implications of mammalian ferritin-binding proteins interacting with circulating ferritin and a new aspect of ferritin- and zinc-binding proteins.
Orino K.

Front Vet Sci. 2015 May 26;2:12. doi: 10.3389/fvets.2015.00012.
Iron and Ferritin Levels in the Serum and Milk of Bovine Leukemia Virus-Infected Dairy Cows.
Schnell SA, Ohtsuka H, Kakinuma S, Yoshikawa Y, Watanabe K, Orino K.

Vet J. 2015 Nov;206(2):143-8. doi: 10.1016/j.tvjl.2015.08.002.
Molecular cloning of canine co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) and investigation of its ability to suppress androgen receptor signalling in androgen-independent prostate cancer.
Kato Y, Ochiai K, Michishita M, Azakami D, Nakahira R, Morimatsu M, Ishiguro-Oonuma T, Yoshikawa Y, Kobayashi M, Bonkobara M, Kobayashi M, Takahashi K, Watanabe M, Omi T.

Cell Rep. 2015 Mar 31;10(12):1947-56. doi: 10.1016/j.celrep.2015.02.053.
UHRF1 is a sensor for DNA interstrand crosslinks and recruits FANCD2 to initiate the Fanconi anemia pathway.
Liang CC, Zhan B, Yoshikawa Y, Haas W, Gygi SP, Cohn MA. (Co-first author)

BMC Vet Res. 2015 Jul 23;11:159. doi: 10.1186/s12917-015-0483-9.
Reduced canine BRCA2 expression levels in mammary gland tumors.
Yoshikawa Y, Morimatsu M, Ochiai K, Ishiguro-Oonuma T, Wada S, Orino K, Watanabe K.

Biometals. 2015 Aug;28(4):679-85. doi: 10.1007/s10534-015-9853-9.
Analysis of the binding of bovine and human fibrinogen to ferritin: evidence that fibrinogen is a common ferritin-binding protein in mammals.
Okada A, Yoshikawa Y, Watanabe K, Orino K.

Biomed Res. 2015;36(2):155-8. doi: 10.2220/biomedres.36.155.
Polymorphisms of canine BRCA2 BRC repeats affecting interaction with RAD51.
Ochiai K, Ishiguro-Oonuma T, Yoshikawa Y, Udagawa C, Kato Y, Watanabe M, Bonkobara M, Morimatsu M, Omi T.

FEBS J. 2013 Dec;280(24):6600-12. doi: 10.1111/febs.12565.
Autotaxin as a novel, tissue-remodeling-related factor in regressing corpora lutea of cycling rats.
Masuda K, Haruta S, Orino K, Kawaminami M, Kurusu S.

Acta Vet Scand. 2013 Sep 22;55:70. doi: 10.1186/1751-0147-55-70.
The presence of heat-labile factors interfering with binding analysis of fibrinogen with ferritin in horse plasma.
Takahashi K, Kondo T, Yoshikawa Y, Watanabe K, Orino K.

J Vet Med Sci. 2013 Nov;75(11):1419-26. doi: 10.1292/jvms.13-0133.
Change in serum ferritin concentration in experimentally induced anemia of chronic inflammation in dogs.
Chikazawa S, Nakazawa T, Hori Y, Hoshi F, Kanai K, Ito N, Orino K, Watanabe K, Higuchi S.

J Vet Med Sci. 2013 Dec;75(12):1615-8. doi: 10.1292/jvms.13-0200.
Factors influencing measurement of serum iron concentration in dogs: diurnal variation and hyperferritinemia.
Chikazawa S, Hori Y, Kanai K, Ito N, Hoshi F, Orino K, Watanabe K, Higuchi S.

Anim Sci J. 2013 Jul;84(7):556-61. doi: 10.1111/asj.12034.
Measurement of ferritin and anti-ferritin autoantibodies in serum and colostrum of Holstein and Japanese Black cows.
Kanno Y, Ohtsuka H, Yoshikawa Y, Watanabe K, Orino K.

Mol Biol Rep. 2013 Dec;40(12):6747-55. doi: 10.1007/s11033-013-2791-0.
Selection of suitable reference genes for mRNA quantification studies using common marmoset tissues.
Shimamoto Y, Kitamura H, Niimi K, Yoshikawa Y, Hoshi F, Ishizuka M, Takahashi E.

J Vet Med Sci. 2013;75(8):1101-5. doi: 10.1292/jvms.12-0561.
Binding analysis of ferritin with heme using α-casein and biotinylated-hemin: detection of heme-binding capacity of Dpr derived from heme synthesis-deficient Streptococcus mutans.
Mieno A, Yamamoto Y, Yoshikawa Y, Watanabe K, Mukai T, Orino K.

J Vet Med Sci. 2013 May;75(4):515-7. doi: 10.1292/jvms.12-0443.
Development of a sandwich enzyme-linked immunosorbent assay to detect and measure serum levels of canine ferritin.
Chikazawa S, Hori Y, Hoshi F, Kanai K, Ito N, Sato J, Orino K, Watanabe K, Higuchi S.

Vet J. 2013 Sep;197(3):769-75. doi: 10.1016/j.tvjl.2013.04.024.
Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.
Ochiai K, Watanabe M, Azakami D, Michishita M, Yoshikawa Y, Udagawa C, Metheenukul P, Chahomchuen T, Aoki H, Kumon H, Morimatsu M, Omi T.

Anim Sci J. 2013 Dec;84(12):782-9. doi: 10.1111/asj.12069.
Change of antibody levels to ferritin in the sera of foals after birth: possible passive transfer of maternal anti-ferritin autoantibody via colostrum and age-related anti-ferritin autoantibody production.
Numata M, Kondo T, Nambo Y, Yoshikawa Y, Watanabe K, Orino K.

PLoS One. 2012;7(10):e45833. doi: 10.1371/journal.pone.0045833.
Effects of the missense mutations in canine BRCA2 on BRC repeat 3 functions and comparative analyses between canine and human BRC repeat 3.
Yoshikawa Y, Ochiai K, Morimatsu M, Suzuki Y, Wada S, Taoda T, Iwai S, Chikazawa S, Orino K, Watanabe K.

Biometals. 2012 Oct;25(5):1083-8. doi: 10.1007/s10534-012-9573-3.
Iron-dependent binding of bovine milk α-casein with holo-lactoferrin, but not holo-transferrin.
Shibuya N, Yoshikawa Y, Watanabe K, Ohtsuka H, Orino K.

BMC Res Notes. 2012 Apr 3;5:173. doi: 10.1186/1756-0500-5-173.
Establishment of a PCR analysis method for canine BRCA2.
Yoshikawa Y, Morimatsu M, Ochiai K, Okuda K, Taoda T, Chikazawa S, Shimamura A, Omi T, Bonkobara M, Orino K, Watanabe K.

J Vet Med Sci. 2012 May;74(5):583-9. doi: 10.1292/jvms.11-0392.
Effects of oxidative stress caused by tert-butylhydroquinone on cytotoxicity in MDCK cells.
Shibuya N, Kobayashi S, Yoshikawa Y, Watanabe K, Orino K.

Biometals. 2011 Dec;24(6):1217-24. doi: 10.1007/s10534-011-9470-1.
Heme-mediated binding of α-casein to ferritin: evidence for preferential α-casein binding to ferrous iron.
Usami A, Tanaka M, Yoshikawa Y, Watanabe K, Ohtsuka H, Orino K.

Biometals. 2011 Dec;24(6):1123-31. doi: 10.1007/s10534-011-9471-0.
Effect of diquat-induced oxidative stress on iron metabolism in male Fischer-344 rats.
Higuchi M, Yoshikawa Y, Orino K, Watanabe K.

Poult Sci. 2011 Jul;90(7):1489-95. doi: 10.3382/ps.2010-01307.
Structural and functional analyses of chicken liver ferritin.
Watanabe M, Yuge M, Uda A, Yoshikawa Y, Watanabe K, Orino K.

Biosci Biotechnol Biochem. 2011;75(3):496-9. doi: 10.1271/bbb.100754.
Wheat bran protects Fischer-344 rats from diquat-induced oxidative stress by activating antioxidant system: selenium as an antioxidant.
Higuchi M, Oshida J, Orino K, Watanabe K.

FEBS Lett. 2011 Jun 23;585(12):1771-7. doi: 10.1016/j.febslet.2011.05.027.
Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51.
Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu M.

J Vet Med Sci. 2011 Mar;73(3):313-8. doi: 10.1292/jvms.10-0243.
Binding of mammalian and avian ferritins with biotinylated hemin: demonstration of preferential binding of the H subunit to heme.
Nakai M, Murata N, Yoda T, Yoshikawa Y, Watanabe K, Orino K.